Solid-phase competitive and sandwich-type erythro-immunoassays for human chorionic gonadotropin.

@article{Gupta1985SolidphaseCA,
  title={Solid-phase competitive and sandwich-type erythro-immunoassays for human chorionic gonadotropin.},
  author={Sanjeev K. Gupta and Jean Luc Guesdon and Stratis Avrameas and Gursaran Pran Talwar},
  journal={Journal of immunological methods},
  year={1985},
  volume={80 2},
  pages={
          177-87
        }
}
A simple '1-step' competitive erythro-immunoassay for human chorionic gonadotropin (hCG) employing V-shaped well microtitration plates coated with monoclonal anti-beta-hCG antibody has been described. hCG of the test sample competes with the antigen-coupled sheep erythrocytes for binding to the antibody on the solid surface. The assay is able to detect up to 31.25 ng hCG/ml. A higher sensitivity enabling detection up to 0.25 ng hCG/ml is attained by the sandwich erythro-immunoassay using a… CONTINUE READING
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