Soil eukaryotic functional diversity, a metatranscriptomic approach

  title={Soil eukaryotic functional diversity, a metatranscriptomic approach},
  author={Julie Bailly and Laurence Fraissinet-Tachet and M C Verner and J Debaud and Marc Lemaire and Micheline W{\'e}solowski-Louvel and Roland Marmeisse},
  journal={The ISME Journal},
To appreciate the functional diversity of communities of soil eukaryotic micro-organisms we evaluated an experimental approach based on the construction and screening of a cDNA library using polyadenylated mRNA extracted from a forest soil. Such a library contains genes that are expressed by each of the different organisms forming the community and represents its metatranscriptome. The diversity of the organisms that contributed to this library was evaluated by sequencing a portion of the 18S… CONTINUE READING

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