Soft Substrates Promote Homogeneous Self-Renewal of Embryonic Stem Cells via Downregulating Cell-Matrix Tractions

@inproceedings{Chowdhury2010SoftSP,
  title={Soft Substrates Promote Homogeneous Self-Renewal of Embryonic Stem Cells via Downregulating Cell-Matrix Tractions},
  author={Farhan Chowdhury and Yanzhen Li and Y P Poh and Tamaki Yokohama-Tamaki and Ning Wang and Tetsuya S. Tanaka},
  booktitle={PloS one},
  year={2010}
}
Maintaining undifferentiated mouse embryonic stem cell (mESC) culture has been a major challenge as mESCs cultured in Leukemia Inhibitory Factor (LIF) conditions exhibit spontaneous differentiation, fluctuating expression of pluripotency genes, and genes of specialized cells. Here we show that, in sharp contrast to the mESCs seeded on the conventional rigid substrates, the mESCs cultured on the soft substrates that match the intrinsic stiffness of the mESCs and in the absence of exogenous LIF… CONTINUE READING
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