Sodium ascorbate induces DNA single-strand breaks in human cells in vitro.

@article{Singh1997SodiumAI,
  title={Sodium ascorbate induces DNA single-strand breaks in human cells in vitro.},
  author={Narendra Pal Singh},
  journal={Mutation research},
  year={1997},
  volume={375 2},
  pages={195-203}
}
Incubation of human lymphocytes, neonatal fibroblasts, and Molt-4 cells (T-cell leukemia cell line) with sodium ascorbate for 1 h resulted in a concentration-dependent increase in DNA single-strand breaks as assayed by an alkaline microgel electrophoresis technique. Fibroblasts and Molt-4 cells were significantly more sensitive than lymphocytes to the induction of DNA single-strand breaks by 25, 50, and 100 microM concentrations of sodium ascorbate. Significant cell loss was observed in Molt-4… CONTINUE READING