Sodium arsenite-induced inhibition of cell proliferation is related to inhibition of IL-2 mRNA expression in mouse activated T cells

Abstract

A proposed mechanism for the As-induced inhibition of cell proliferation is the inhibition of IL-2 secretion. However, the effects of arsenite on IL-2 mRNA expression or on the ERK pathway in activated-T cells have not yet been described. We examined the effect of arsenite on IL-2 mRNA expression, cell activation and proliferation in PHA-stimulated murine lymphocytes. Arsenite (1 and 10 μM) decreased IL-2 mRNA expression, IL-2 secretion and cell proliferation. Arsenite (10 μM) strongly inhibited ERK-phosphorylation. However, the partial inhibition (50%) of IL-2 mRNA produced by 1 μM, consistent with the effects on IL-2 secretion and cell proliferation, could not be explained by the inhibition of ERK-phosphorylation, which was not affected at this concentration. The inhibition of IL-2 mRNA expression caused by 1 μM could be associated to effects on pathways located downstream or parallel to ERK. Arsenite also decreased early activation (surface CD69+ expression) in both CD4+ and CD8+, and decreased total CD8+ count without significantly affecting CD4+, supporting that the cellular immune response mediated by cytotoxic T cells is an arsenic target. Thus, our results suggest that arsenite decreases IL-2 mRNA levels and T-cell activation and proliferation. However, further studies on the effects of arsenite on IL-2 gene transcription and IL-2 mRNA stability are needed.

DOI: 10.1007/s00204-006-0152-7

6 Figures and Tables

02004006002008200920102011201220132014201520162017
Citations per Year

1,150 Citations

Semantic Scholar estimates that this publication has 1,150 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Conde2006SodiumAI, title={Sodium arsenite-induced inhibition of cell proliferation is related to inhibition of IL-2 mRNA expression in mouse activated T cells}, author={Patricia Conde and Leonor C Acosta-Saavedra and Raquel C. Goytia-Acevedo and Emma S Calder{\'o}n-Aranda}, journal={Archives of Toxicology}, year={2006}, volume={81}, pages={251-259} }