Snapback primer genotyping with saturating DNA dye and melting analysis.

@article{Zhou2008SnapbackPG,
  title={Snapback primer genotyping with saturating DNA dye and melting analysis.},
  author={Luming Zhou and Roscoe J Errigo and Hongzhe Lu and Mark A. Poritz and Michael T. Seipp and Carl T. Wittwer},
  journal={Clinical chemistry},
  year={2008},
  volume={54 10},
  pages={
          1648-56
        }
}
BACKGROUND DNA hairpins have been used in molecular analysis of PCR products as self-probing amplicons. Either physical separation or fluorescent oligonucleotides with covalent modifications were previously necessary. METHODS We performed asymmetric PCR for 40-45 cycles in the presence of the saturating DNA dye, LCGreen Plus, with 1 primer including a 5' tail complementary to its extension product, but without any special covalent modifications. Samples were amplified either on a carousel… 

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