Snapback primer genotyping with saturating DNA dye and melting analysis.

  title={Snapback primer genotyping with saturating DNA dye and melting analysis.},
  author={L. Zhou and Roscoe J Errigo and Hongzhe Lu and M. Poritz and M. Seipp and C. Wittwer},
  journal={Clinical chemistry},
  volume={54 10},
  • L. Zhou, Roscoe J Errigo, +3 authors C. Wittwer
  • Published 2008
  • Chemistry, Medicine
  • Clinical chemistry
  • BACKGROUND DNA hairpins have been used in molecular analysis of PCR products as self-probing amplicons. Either physical separation or fluorescent oligonucleotides with covalent modifications were previously necessary. METHODS We performed asymmetric PCR for 40-45 cycles in the presence of the saturating DNA dye, LCGreen Plus, with 1 primer including a 5' tail complementary to its extension product, but without any special covalent modifications. Samples were amplified either on a carousel… CONTINUE READING

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