Smooth-muscle contraction without smooth-muscle myosin

  title={Smooth-muscle contraction without smooth-muscle myosin},
  author={Ingo Morano and Gui-Xuan Chai and Leonidas G. Baltas and Val{\'e}ria Lamounier-Zepter and Gudrun Lutsch and Monika Kott and Hannelore Haase and Michael Bader},
  journal={Nature Cell Biology},
Here we have used gene-targeting to eliminate expression of smooth-muscle myosin heavy chain. Elimination of this gene does not affect expression of non-muscle myosin heavy chain, and knockout individuals typically survive for three days. Prolonged activation, by KCl depolarisation, of intact bladder preparations from wild-type neonatal mice produces an initial transient state (phase 1) of high force generation and maximal shortening velocity, which is followed by a sustained state (phase 2… CONTINUE READING