Smooth, cardiac and skeletal muscle myosin force and motion generation assessed by cross-bridge mechanical interactions in vitro

@article{Harris1994SmoothCA,
  title={Smooth, cardiac and skeletal muscle myosin force and motion generation assessed by cross-bridge mechanical interactions in vitro},
  author={David Eisenbud Joe Harris and Steven S. Work and Reba K. Wright and Norman R. Alpert and David M. Warshaw},
  journal={Journal of Muscle Research & Cell Motility},
  year={1994},
  volume={15},
  pages={11-19}
}
Differences in the mechanical properties of mammalian smooth, skeletal, and cardiac muscle have led to the proposal that the myosin isozymes expressed by these tissues may differ in their molecular mechanics. To test this hypothesis, mixtures of fast skeletal, V1 cardiac, V3 cardiac and smooth muscle (phosphorylated and unphosphorylated) myosin were studied in an in vitro motility assay in which fluorescently-labelled actin filaments are observed moving over a myosin coated surface. Pure… CONTINUE READING