Smokers and passive smokers gene expression profiles: correlation with the DNA oxidation damage.

@article{Lodovici2007SmokersAP,
  title={Smokers and passive smokers gene expression profiles: correlation with the DNA oxidation damage.},
  author={Maura Lodovici and Cristina Luceri and Carlotta De Filippo and Chiara Romualdi and Franco Bambi and Piero Dolara},
  journal={Free radical biology & medicine},
  year={2007},
  volume={43 3},
  pages={415-22}
}
Healthy volunteers (n=50) were enrolled for studying the variation of gene expression induced by smoking in peripheral lymphocytes. RNAs from smokers (>3 cigarettes/day, n=20) and passive smokers (exposed to tobacco smoke >3 h/day, n=10) were hybridized versus a reference pool obtained by mixing equal amounts of RNA from 20 nonsmokers, and gene expression was analyzed using DNA microarrays containing 13,971 oligos. Principal component analysis showed that 99.7% of gene expression variability… CONTINUE READING