Smart hydrogel particles: biomarker harvesting: one-step affinity purification, size exclusion, and protection against degradation.

@article{Luchini2008SmartHP,
  title={Smart hydrogel particles: biomarker harvesting: one-step affinity purification, size exclusion, and protection against degradation.},
  author={Alessandra Luchini and David H Geho and Barney M Bishop and Duy Tran and Cassandra Xia and Robert L Dufour and Clinton D Jones and Virginia Espina and Alexis S Patanarut and Weidong Zhou and Mark Ross and Alessandra Tessitore and Emanual F. Petricoin and Lance A. Liotta},
  journal={Nano letters},
  year={2008},
  volume={8 1},
  pages={350-61}
}
Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The… CONTINUE READING
37 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 37 extracted citations

Similar Papers

Loading similar papers…