Small variations in crucial steps of TUNEL assay coupled to flow cytometry greatly affect measures of sperm DNA fragmentation.

@article{Muratori2010SmallVI,
  title={Small variations in crucial steps of TUNEL assay coupled to flow cytometry greatly affect measures of sperm DNA fragmentation.},
  author={Monica Muratori and Lara Tamburrino and Valentina Tocci and Antonietta Costantino and Sara Marchiani and Claudia Giachini and Ilaria Laface and C Krausz and Maria C Meriggiola and Gianni Forti and Elisabetta Baldi},
  journal={Journal of andrology},
  year={2010},
  volume={31 4},
  pages={336-45}
}
Techniques for assessing sperm DNA damage are numerous and various. There are 2 main types of assay: direct and indirect. The former directly detects the amount of sperm DNA damage, whereas the latter reveals the effects of an exogenous insult on sperm chromatin. In addition, even considering the same type of technique, different strategies to reveal or quantify sperm DNA damage, or both, are used. Finally, these techniques, except for sperm chromatin structure assay (SCSA), lack standardized… CONTINUE READING

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Human sperm DNA fragmentation: correlation of TUNEL results as assessed by flow cytometry and optical microscopy.

Cytometry. Part A : the journal of the International Society for Analytical Cytology • 2007
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