Small unilamellar vesicles containing glycophorin A. Chemical characterization and proton nuclear magnetic resonance studies.

@article{Ong1981SmallUV,
  title={Small unilamellar vesicles containing glycophorin A. Chemical characterization and proton nuclear magnetic resonance studies.},
  author={Richard L. Ong and Vincent T. Marchesi and James H. Prestegard},
  journal={Biochemistry},
  year={1981},
  volume={20 15},
  pages={4283-92}
}
Glycophorin A, a major glycoprotein of the red blood cell, is reconstituted in small lipid vesicles (250-300 A in diameter) by using cholate detergent solubilization followed by rapid removal of cholate on a molecular sieve column. The extent of glycophorin incorporation is found to be critically dependent on the amount of cholate used, with higher amounts yielding vesicles with higher percentages of glycophorin. Vesicles with as much as 1 molecule of protein per 20 molecules of lipid can be… CONTINUE READING

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