Small-seq for single-cell small-RNA sequencing

  title={Small-seq for single-cell small-RNA sequencing},
  author={Michael Hagemann-Jensen and I. Abdullayev and R. Sandberg and Omid R Faridani},
  journal={Nature Protocols},
  • Michael Hagemann-Jensen, I. Abdullayev, +1 author Omid R Faridani
  • Published 2018
  • Chemistry, Medicine
  • Nature Protocols
  • Small RNAs participate in several cellular processes, including splicing, RNA modification, mRNA degradation, and translational arrest. Traditional methods for sequencing small RNAs require a large amount of cell material, limiting the possibilities for single-cell analyses. We describe Small-seq, a ligation-based method that enables the capture, sequencing, and molecular counting of small RNAs from individual mammalian cells. Here, we provide a detailed protocol for this approach that relies… CONTINUE READING
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