Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells

@inproceedings{Li2017SmallME,
  title={Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells},
  author={Guoling Li and Xianwei Zhang and Cuili Zhong and Jianxin Mo and Rong Quan and Jie Yang and Dewu Liu and Zicong Li and Huaqiang Yang and Zhenfang Wu},
  booktitle={Scientific Reports},
  year={2017}
}
CRISPR/Cas9 is an efficient customizable nuclease to generate double-strand breaks (DSBs) in the genome. This process results in knockout of the targeted gene or knock-in of a specific DNA fragment at the targeted locus in the genome of various species. However, efficiency of knock-in mediated by homology-directed repair (HDR) pathway is substantially lower compared with the efficiency of knockout mediated by the nonhomologous end-joining (NHEJ) pathway. Suppressing NHEJ pathway or enhancing… CONTINUE READING
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