Small molecule inhibitors of phospholipase C from a novel high-throughput screen.

@article{Huang2013SmallMI,
  title={Small molecule inhibitors of phospholipase C from a novel high-throughput screen.},
  author={Weigang Huang and Matthew O Barrett and Nicole Hajicek and Stephanie N. Hicks and T. Kendall Harden and John E Sondek and Qisheng Zhang},
  journal={The Journal of biological chemistry},
  year={2013},
  volume={288 8},
  pages={5840-8}
}
Phospholipase C (PLC) isozymes are important signaling molecules, but few small molecule modulators are available to pharmacologically regulate their function. With the goal of developing a general approach for identification of novel PLC inhibitors, we developed a high-throughput assay based on the fluorogenic substrate reporter WH-15. The assay is highly sensitive and reproducible: screening a chemical library of 6280 compounds identified three novel PLC inhibitors that exhibited potent… CONTINUE READING

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