Small molecule inhibitor of lipoteichoic acid synthesis is an antibiotic for Gram-positive bacteria

@article{Richter2013SmallMI,
  title={Small molecule inhibitor of lipoteichoic acid synthesis is an antibiotic for Gram-positive bacteria},
  author={Stefan Richter and Derek Elli and Hwan Keun Kim and Antoni P A Hendrickx and Joseph A. Sorg and Olaf Schneewind and Dominique Missiakas},
  journal={Proceedings of the National Academy of Sciences},
  year={2013},
  volume={110},
  pages={3531 - 3536}
}
The current epidemic of infections caused by antibiotic-resistant Gram-positive bacteria requires the discovery of new drug targets and the development of new therapeutics. Lipoteichoic acid (LTA), a cell wall polymer of Gram-positive bacteria, consists of 1,3-polyglycerol-phosphate linked to glycolipid. LTA synthase (LtaS) polymerizes polyglycerol-phosphate from phosphatidylglycerol, a reaction that is essential for the growth of Gram-positive bacteria. We screened small molecule libraries for… Expand
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TLDR
An in vitro assay for this new class of enzyme using PG with a fluorescently labeled fatty acid chain as the substrate and the recombinant soluble C-terminal enzymatic domain of LtaS revealed that eLtaS is sufficient to cleave the glycerolphosphate head group from NBD-PG, resulting in the formation of N BD-diacylglycerol. Expand
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TLDR
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The presented results indicate that the mere presence or absence of LTA cannot account for cell division and sporulation defects observed in the absence of individual enzymes and revealed an unexpected enzymatic interdependency of LtaS‐type proteins in B. subtilis. Expand
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TLDR
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