Small molecule gated split-tyrosine phosphatases and orthogonal split-tyrosine kinases.

@article{CamachoSoto2014SmallMG,
  title={Small molecule gated split-tyrosine phosphatases and orthogonal split-tyrosine kinases.},
  author={Karla Camacho-Soto and Javier Castillo-Montoya and Blake Tye and Luca O Ogunleye and Indraneel Ghosh},
  journal={Journal of the American Chemical Society},
  year={2014},
  volume={136 49},
  pages={17078-86}
}
Protein kinases phosphorylate client proteins, while protein phosphatases catalyze their dephosphorylation and thereby in concert exert reversible control over numerous signal transduction pathways. We have recently reported the design and validation of split-protein kinases that can be conditionally activated by an added small molecule chemical inducer of dimerization (CID), rapamycin. Herein, we provide the rational design and validation of three split-tyrosine phosphatases (PTPs) attached to… CONTINUE READING