Small-molecule-dependent split aptamer ligation.

@article{Sharma2011SmallmoleculedependentSA,
  title={Small-molecule-dependent split aptamer ligation.},
  author={Ashwani K. Sharma and Jennifer M Heemstra},
  journal={Journal of the American Chemical Society},
  year={2011},
  volume={133 32},
  pages={
          12426-9
        }
}
Here we describe the first use of small-molecule binding to direct a chemical reaction between two nucleic acid strands. The reported reaction is a ligation between two fragments of a DNA split aptamer using strain-promoted azide-alkyne cycloaddition. Utilizing the split aptamer for cocaine, we demonstrate small-molecule-dependent ligation that is dose-dependent over a wide range of cocaine concentrations and is compatible with complex biological fluids such as human blood serum. Moreover… CONTINUE READING

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