Small is big in Arabidopsis mitochondrial ribosome

@article{Waltz2019SmallIB,
  title={Small is big in Arabidopsis mitochondrial ribosome},
  author={Florent Waltz and Tan-Trung Nguyen and Mathilde Arriv{\'e} and Anthony Bochler and Johana Chicher and Philippe Hammann and Lauriane Kuhn and Martine Quadrado and Hakim Mireau and Yaser Hashem and Philippe Gieg{\'e}},
  journal={Nature Plants},
  year={2019},
  volume={5},
  pages={106-117}
}
Mitochondria are responsible for energy production through aerobic respiration, and represent the powerhouse of eukaryotic cells. Their metabolism and gene expression processes combine bacterial-like features and traits that evolved in eukaryotes. Among mitochondrial gene expression processes, translation remains the most elusive. In plants, while numerous pentatricopeptide repeat (PPR) proteins are involved in all steps of gene expression, their function in mitochondrial translation remains… 

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References

SHOWING 1-10 OF 82 REFERENCES

A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression

A systematic study of the PPR proteins in the fission yeast Schizosaccharomyces pombe identified eight proteins all of which localized to the mitochondria, and showed some association with the membrane, leading to a respiratory deficiency and modified patterns of steady state mt-mRNAs or newly synthesized mitochondrial proteins.

The translational landscape of Arabidopsis mitochondria

Observations reveal that plant mitochondrial translation is a dynamic process and that translational control is important for gene expression in plant mitochondria, and paves the way for future advances in the understanding translation in higher plant mitochondia.

The MTL1 Pentatricopeptide Repeat Protein Is Required for Both Translation and Splicing of the Mitochondrial NADH DEHYDROGENASE SUBUNIT7 mRNA in Arabidopsis1

It is demonstrated that mtl1 mutant plants fail to accumulate the Nad7 protein, even though the nad7 mature mRNA is produced and bears the same 5′ and 3′ extremities as in wild-type plants, illustrating that mitochondrial translation requires the intervention of gene-specific nucleus-encoded PPR trans-factors and that their action does not necessarily involve the 5′ processing of their target mRNA.

Mitochondrial ribosome assembly in health and disease

This article focuses on summarizing the current available information on mitoribosome assembly pathway, factors that form the mitoribergsome assembly machinery, and the effect of defective mitorIBosomeAssembly assembly on human health.

Organization and Regulation of Mitochondrial Protein Synthesis.

This review places the new structural information in the context of the molecular mechanisms of mitochondrial translation and focus on the novel ways protein synthesis is organized and regulated in mitochondria.

Structure of the Yeast Mitochondrial Large Ribosomal Subunit

The structure of the yeast mitoribosomal large subunit is solved using single-particle cryo–electron microscopy and reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.

An Arabidopsis Dual-Localized Pentatricopeptide Repeat Protein Interacts with Nuclear Proteins Involved in Gene Expression Regulation[W]

PNM1, a novel PPR protein that is dual localized to mitochondria and nuclei in Arabidopsis thaliana, is identified, as observed by green fluorescent protein fusions and immunodetection on subcellular fractions and on histological sections, which suggests that PNM1 might be involved in the regulation of its own gene expression in the nucleus and could thus play a role in gene expression adjustments between mitochondia and the nucleus.

The complete structure of the 55S mammalian mitochondrial ribosome

The structure reveals the intersubunit contacts in the 55S mitoribosome, the molecular architecture of the mitorIBosomal messenger RNA binding channel and its interaction with transfer RNAs, and provides insight into the highly specialized mechanism of mRNA recruitment to the 28S subunit.

Comparative analysis of bacterial-origin genes for plant mitochondrial ribosomal proteins.

It is found that genes that were transferred to the nucleus early in eukaryotic evolution have, on average, about twofold higher density of introns within the core ribosomal protein sequences than do those that moved to the centre of the nucleus more recently.

The complete structure of the chloroplast 70S ribosome in complex with translation factor pY

The atomic structure of the chloroplast 70S ribosome prepared from spinach leaves and resolved by cryo‐EM at 3.4 Å resolution reveals the features of the 4.5S rRNA, which probably evolved by the fragmentation of the 23S r RNA, and all five plastid‐specific ribosomal proteins.
...