Small interfering RNAs expressed from a Pol III promoter suppress the EWS/Fli-1 transcript in an Ewing sarcoma cell line.

@article{Dohjima2003SmallIR,
  title={Small interfering RNAs expressed from a Pol III promoter suppress the EWS/Fli-1 transcript in an Ewing sarcoma cell line.},
  author={Taikoh Dohjima and Nan Sook Lee and Haitang Li and Takatoshi Ohno and John J. Rossi},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2003},
  volume={7 6},
  pages={811-6}
}
The EWS/Fli-1 fusion gene encodes an oncogenic fusion protein. The fusion is a product of the translocation t(11;22) (q24;q12), which is detected in 85% of Ewing sarcoma and primitive neuroectodermal tumor cells. Utilizing intracellularly expressed 21- to 23-nucleotide small interfering RNAs (siRNAs) targeting the EWS/Fli-1 fusion transcript in an Ewing sarcoma cell line, we achieved a greater than 80% reduction in the EWS/Fli-1 transcript. The reduction in transcript levels was accompanied by… CONTINUE READING