Small interfering RNA of alkaline phosphatase inhibits matrix mineralization

@article{Kotobuki2008SmallIR,
  title={Small interfering RNA of alkaline phosphatase inhibits matrix mineralization},
  author={Noriko Kotobuki and Asako Matsushima and Youichi Kato and Yoko Kubo and Motohiro Hirose and Hajime Ohgushi},
  journal={Cell and Tissue Research},
  year={2008},
  volume={332},
  pages={279-288}
}
To investigate the cascade of matrix mineralization, cells expressing high and low alkaline phosphatase (ALP) were separated from human osteoblast-like (HOS) cells by fluorescence-activated cell sorting with an ALP antibody. After these cells had been recloned from single cells and then cultured under osteogenic conditions, high-ALP-expressing HOS (H-HOS… CONTINUE READING