Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae

@article{Funakoshi2009SmallEM,
  title={Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae},
  author={Minoru Funakoshi and Mark Hochstrasser},
  journal={Yeast (Chichester, England)},
  year={2009},
  volume={26},
  pages={185 - 192}
}
PCR-mediated gene modification is a powerful approach to the functional analysis of genes in Saccharomyces cerevisiae. One application of this method is epitope-tagging of a gene to analyse the corresponding protein by immunological methods. However, the number of epitope tags available in a convenient format is still low, and interference with protein function by the epitope, particularly if it is large, is not uncommon. To address these limitations and broaden the utility of the method, we… CONTINUE READING

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