Small RNAs couple embryonic developmental programs to gut microbes

  title={Small RNAs couple embryonic developmental programs to gut microbes},
  author={Hayao Ohno and Zhirong Bao},
Maternal exposure to microbes and other environmental factors is known to induce adaptive changes in the progeny, but little is understood about how development of the progeny is changed. We show that Caenorhabditis elegans undergoes additional embryonic cell divisions in response to maternal gut microbes such as one producing the biopolymer γ-poly-DL-glutamic acid. The divisions coincide with anatomical changes including left-right asymmetric cell alignment, doubling the association between… Expand
1 Citations
Intercellular transport of RNA can limit heritable epigenetic changes
Evidence is provided for spatial, temporal, and substrate specificity in the transport of dsRNA from parental circulation to progeny to limit heritable changes in gene expression. Expand


AMPK regulates germline stem cell quiescence and integrity through an endogenous small RNA pathway
These findings place AMPK at a pivotal position linking energy stress detected in the soma to a consequent endogenous small RNA–mediated adaptation in germline gene expression, thereby challenging the “permeability" of the Weismann barrier. Expand
Neuronal Small RNAs Control Behavior Transgenerationally
It is shown that a neuronal process can impact the next generations, and a small-RNA-based mechanism for communication of neuronal processes transgenerationally is proposed. Expand
Small RNA in situ hybridization in Caenorhabditis elegans, combined with RNA-seq, identifies germline-enriched microRNAs☆
A successful miRNA in situ hybridization protocol is developed for C. elegans, revealing miR35s specifically concentrate during oogenesis in the pachytene region of the gonad, and persist throughout early embryogenesis, while in adult animals neither let-7 nor miR-228 has a germline-bias. Expand
The Embryonic mir-35 Family of microRNAs Promotes Multiple Aspects of Fecundity in Caenorhabditis elegans
It is demonstrated that robust activity of the mir-35-42 family microRNAs not only is essential for embryonic development across a range of temperatures but also enables the worm to subsequently develop full reproductive capacity. Expand
mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C. elegans
It is shown that mir-35 regulates the G1/S transition of intestinal cells and germ cell proliferation and targets evolutionally conserved lin-23 and gld-1 in C. elegans. Expand
C. elegans interprets bacterial non-coding RNAs to learn pathogenic avoidance.
It is shown that a single exposure to purified small RNAs isolated from pathogenic Pseudomonas aeruginosa (PA14) is sufficient to induce pathogen avoidance in the treated worms and in four subsequent generations of progeny. Expand
Biology and Mechanisms of Short RNAs in Caenorhabditis elegans.
  • A. Grishok
  • Biology, Medicine
  • Advances in genetics
  • 2013
This chapter provides a broad overview of the exogenous and endogenous RNAi processes in C. elegans and describes recent advances in genetic, genomic, and molecular analyses of nematode's short RNAs and proteins involved in the RNAi-related pathways. Expand
A Complex Regulatory Network Coordinating Cell Cycles During C. elegans Development Is Revealed by a Genome-Wide RNAi Screen
A genome-wide RNA interference screen in Caenorhabditis elegans revealed the components of a regulatory network that promotes developmentally programmed cell-cycle quiescence and characterized ubc-25, a gene encoding an E2 ubiquitin-conjugating enzyme whose human ortholog, UBE2Q2, is deregulated in several cancers. Expand
CDC-25.1 stability is regulated by distinct domains to restrict cell division during embryogenesis in C. elegans
The characterization of a novel destabilizing cdc-25.1(lf) intragenic suppressor that acts independently of lin-23 indicates that additional cues impinge on different motifs of the CDC- 25.1 phosphatase during early embryogenesis to ensure the timely divisions of intestinal cells and coordinate them with the formation of the developing gut. Expand
Many Families of C. elegans MicroRNAs Are Not Essential for Development or Viability
The findings indicate that the regulatory functions of most individual miRNAAs and most individual families of miRNAs related in sequence are not critical for development or viability, and emphasizes the importance of determining miRNA function in the absence of miRNA related in sequences. Expand