Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation

@inproceedings{Shore2016SmallRL,
  title={Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation},
  author={Sabrina Shore and Jordana M. Henderson and Alexandre V. Lebedev and Michelle P Salcedo and G. D. R. Zon and Anton P. McCaffrey and Natasha Paul and Richard I. Hogrefe},
  booktitle={PloS one},
  year={2016}
}
For most sample types, the automation of RNA and DNA sample preparation workflows enables high throughput next-generation sequencing (NGS) library preparation. Greater adoption of small RNA (sRNA) sequencing has been hindered by high sample input requirements and inherent ligation side products formed during library preparation. These side products, known as adapter dimer, are very similar in size to the tagged library. Most sRNA library preparation strategies thus employ a gel purification… CONTINUE READING
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