Small Molecule-Assisted, Line-Independent Maintenance of Human Pluripotent Stem Cells in Defined Conditions

@article{Frank2012SmallML,
  title={Small Molecule-Assisted, Line-Independent Maintenance of Human Pluripotent Stem Cells in Defined Conditions},
  author={S. Frank and M. Zhang and H. Sch{\"o}ler and B. Greber},
  journal={PLoS ONE},
  year={2012},
  volume={7}
}
  • S. Frank, M. Zhang, +1 author B. Greber
  • Published 2012
  • Biology, Medicine
  • PLoS ONE
  • Human pluripotent stem cells (hPSCs) are conventionally grown in a mouse feeder cell-dependent manner. Chemically defined culture conditions are, however, desirable not only for potential medically oriented applications but also for investigating mechanisms of self-renewal and differentiation. In light of the rather high complexity and cost of existing defined hPSC culture systems, we have systematically evaluated over 20 potential media ingredients. Only components that reproducibly gave… CONTINUE READING

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