Small Molecule-Assisted, Line-Independent Maintenance of Human Pluripotent Stem Cells in Defined Conditions

@inproceedings{Frank2012SmallML,
  title={Small Molecule-Assisted, Line-Independent Maintenance of Human Pluripotent Stem Cells in Defined Conditions},
  author={S. P. Frank and Miao Zhang and Hans R. Sch{\"o}ler and Boris Greber},
  booktitle={PloS one},
  year={2012}
}
Human pluripotent stem cells (hPSCs) are conventionally grown in a mouse feeder cell-dependent manner. Chemically defined culture conditions are, however, desirable not only for potential medically oriented applications but also for investigating mechanisms of self-renewal and differentiation. In light of the rather high complexity and cost of existing defined hPSC culture systems, we have systematically evaluated over 20 potential media ingredients. Only components that reproducibly gave… CONTINUE READING

6 Figures & Tables

Topics

Statistics

050100201320142015201620172018
Citations per Year

197 Citations

Semantic Scholar estimates that this publication has 197 citations based on the available data.

See our FAQ for additional information.