Small Interfering RNA-Induced Transcriptional Gene Silencing in Human Cells

@article{Morris2004SmallIR,
  title={Small Interfering RNA-Induced Transcriptional Gene Silencing in Human Cells},
  author={Kevin V. Morris and Simon W.-L. Chan and Steven E. Jacobsen and David J. Looney},
  journal={Science},
  year={2004},
  volume={305},
  pages={1289 - 1292}
}
Small interfering RNA (siRNA) and microRNA silence genes at the transcriptional, posttranscriptional, and/or translational level. Using human tissue culture cells, we show that promoter-directed siRNA inhibits transcription of an integrated, proviral, elongation factor 1alpha (EF1A) promoter–green fluorescent protein reporter gene and of endogenous EF1A. Silencing was associated with DNA methylation of the targeted sequence, and it required either active transport of siRNA into the nucleus or… 
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RNA-mediated transcriptional gene silencing in human cells.

  • K. Morris
  • Biology
    Current topics in microbiology and immunology
  • 2008
The observation that siRNA-directed TGS is operative via epigenetic modifications suggests that similar to plants, and S. Pombe, human genes may also be able to be silenced more permanently or for longer periods following a single treatment and may offer a new therapeutic avenue that could prove robust and of immeasurable therapeutic value in the directed control of target gene expression.

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