Slow-channel mutation in acetylcholine receptor alphaM4 domain and its efficient knockdown.

@article{Shen2006SlowchannelMI,
  title={Slow-channel mutation in acetylcholine receptor alphaM4 domain and its efficient knockdown.},
  author={X Shen and Feza Deymeer and Steven M. Sine and Andrew Geddes Engel},
  journal={Annals of neurology},
  year={2006},
  volume={60 1},
  pages={128-36}
}
OBJECTIVE To identify the genetic basis of a slow-channel myasthenic syndrome, characterize functional properties of the mutant receptor, and selectively silence the mutant allele. METHODS We performed nutation analysis, cloning, and patch-clamp analysis of the functional properties of the mutant receptor; screening for a small interfering RNA with check plasmid; and assessed of the efficacy of small interfering RNA at the messenger RNA, protein, and functional levels. RESULTS We traced the… CONTINUE READING

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