Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression.

@article{Score2006SleepingBT,
  title={Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression.},
  author={Paul R Score and Lalitha R. Belur and Joel L Frandsen and Jennifer L. Geurts and Tomoyuki Yamaguchi and Nikunj V. Somia and Perry B. Hackett and David A. Largaespada and R. Scott McIvor},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2006},
  volume={13 3},
  pages={617-24}
}
The Sleeping Beauty transposon system (SB) has been shown to mediate nonviral integration of expression constructs resulting in long-term gene expression in several mammalian targets. Often, however, it is difficult to discern long-term expression resulting from transposition vs nonhomologous chromosomal recombination or maintenance of plasmid DNA in an extrachromosomal form. We have designed a system to silence expression from nontransposed sequences, making it possible to determine more… CONTINUE READING

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