Skin mast cells develop non-synchronized changes in typical lineage characteristics upon culture.

@article{Guhl2014SkinMC,
  title={Skin mast cells develop non-synchronized changes in typical lineage characteristics upon culture.},
  author={Sven Guhl and Angelos Neou and Metin Artuc and Torsten Zuberbier and Magda Babina},
  journal={Experimental dermatology},
  year={2014},
  volume={23 12},
  pages={
          933-5
        }
}
Despite their hematopoietic origin, mast cells (MCs) develop exclusively in tissues, hampering their ample use in research. To circumvent this problem, tissue-derived MCs are typically first expanded in culture, but the changes MCs may undergo in the novel micromilieu are poorly defined. Here, we monitor skin MCs from a number of donors over time, revealing profound yet non-synchronized modulations in culture. While tryptase and chymase, the most specific markers, strongly decline, FcεRI… CONTINUE READING
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