Skeletal muscle regeneration after insulin-like growth factor I gene transfer by recombinant Sendai virus vector


We scrutinized the applicability and efficacy of Sendai virus (SeV) vectors expressing either LacZ or human insulin-like growth factor-I (hIGF-I) in gene transfer into skeletal muscle. Seven days after the intramuscular injection of LacZ/SeV X-gal labeled myofibers were demonstrated in rat anterior tibialis muscle with/without bupivacaine treatment and the transgene expression persisted up to 1 month after injection. Recombinant hIGF-I was detected as a major protein species in culture supernatants of a neonatal rat myoblast cell line L6 and thus induced the cells to undergo myogenetic differentiation. The introduction of hIGF-I/SeV into the muscle showed a significant increase in regenerating and split myofibers which were indicative of hypertrophy, and also an increase in the total number of myofibers, in comparison to that seen in the LacZ/SeV-treated control muscle. These results demonstrate that SeV achieves high-level transgene expression in skeletal muscle, and that hIGF-I gene transfer using SeV vector may therefore have great potential in the treatment of neuromuscular disorders.

DOI: 10.1038/


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@article{Shiotani2001SkeletalMR, title={Skeletal muscle regeneration after insulin-like growth factor I gene transfer by recombinant Sendai virus vector}, author={Akihiro Shiotani and Mihoko Fukumura and M Maeda and X. Q. Hou and Masashi Inoue and Takuya Kanamori and Shintarou Komaba and Kazuhiko Washizawa and Satoru Fujikawa and Tetsuo Yamamoto and Chiho Kadono and Kazuhiko Watabe and Hiroyuki Fukuda and Kiyoshi Saito and Yasuyuki Sakai and Yusuke Nagai and Jin Kanzaki and Masatoshi Hasegawa}, journal={Gene Therapy}, year={2001}, volume={8}, pages={1043-1050} }