Size of hirudin sequence required to fold into an active core domain.

The active core domain of hirudin contains three native disulfides (Cys6-Cys14,Cys16-Cys28, and Cys22-Cys39) and 49 amino acid residues (Hir1-49). This compact structure folds spontaneously, and its folding pathway has been elucidated recently [Chatrenet and Chang (1993) J. Biol. Chem. 268, 20988-20996]. The folding mechanisms of Hir1-35 and Hir1-43 were… CONTINUE READING