Size-exclusion chromatography in multidimensional separation schemes for proteome analysis.

@article{Lecchi2003SizeexclusionCI,
  title={Size-exclusion chromatography in multidimensional separation schemes for proteome analysis.},
  author={Paolo Lecchi and Anand R Gupte and Ricardo E. Perez and Lyubov V Stockert and Fred P. Abramson},
  journal={Journal of biochemical and biophysical methods},
  year={2003},
  volume={56 1-3},
  pages={141-52}
}
Size-exclusion chromatography (SEC) is a separation technique with a relatively low resolving power, compared to those usually utilized in proteomics. Therefore, it is often overlooked in experimental protocols, when the main goal is resolving complex biological mixtures. In this report, we introduce innovative multidimensional schemes for proteomics analysis, in which SEC plays a practical role. Liquid isoelectric focusing (IEF) was combined with SEC, and experimental results were compared to… CONTINUE READING