Size-exclusion HPLC as a sensitive and calibrationless method for complex peptide mixtures quantification.

Abstract

This work describes an original methodology to quantify complex peptide mixtures by size-exclusion high-performance liquid chromatography (SE-HPLC). The methodology was first tested on simulated elutions of peptide mixtures. For this set of experiments, a good estimation of the total peptide concentration was observed (error less than 10 %). Then 30… (More)
DOI: 10.1016/j.jchromb.2015.09.035

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