Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape

@article{Niemel2012SizeAL,
  title={Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape},
  author={T. Niemel{\"a} and Mervi M. Sepp{\"a}nen and Farah Badakshi and V-M. Rokka and J. S. Heslop-Harrison},
  journal={Chromosome Research},
  year={2012},
  volume={20},
  pages={353-361}
}
In spring turnip rape (Brassica rapa L. spp. oleifera), the most promising F1 hybrid system would be the Ogu-INRA CMS/Rf system. A Kosena fertility restorer gene Rfk1, homolog of the Ogura restorer gene Rfo, was successfully transferred from oilseed rape into turnip rape and that restored the fertility in female lines carrying Ogura cms. The trait was, however, unstable in subsequent generations. The physical localization of the radish chromosomal region carrying the Rfk1 gene was investigated… 
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References

SHOWING 1-10 OF 82 REFERENCES
Transfer of the Kosena Rfk1 gene, required in hybrid seed production, from oilseed rape to turnip rape
TLDR
It was a good indication, that turnip rape with Ogura cms can be restored with the Kosena Rfk1 gene, but it was observed that the RfK1 gene needs to be stabilized before its full exploitation in commercial hybrid production is possible.
Physical localization and genetic mapping of the fertility restoration gene Rfo in canola (Brassica napus L.).
TLDR
The objective of this study was to determine the physical location of the Rfo locus in the canola genome using fluorescence in situ hybridization and genetic mapping and establish a basic framework for further physical mapping and manipulation of the gene.
Mapping of the Ogura fertility restorer gene Rfo and development of Rfo allele-specific markers in canola (Brassica napus L.)
TLDR
This study sequenced genomic clones of Rfo amplified from a canola restorer line R2000, licensed from INRA, France, and a Dow AgroSciences non-restorer line Nexera 705 using primers designed from the radish Rfo sequence (GenBank accession AJ550021).
Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide‐repeat protein family
TLDR
The Ogura CMS/Rfo two‐component system is a useful model for investigating nuclear–cytoplasmic interactions, as well as the physiological basis of fertility restoration, and a member of the pentatricopeptide repeat (PPR) family in Arabidopsis.
A new recombined double low restorer line for the Ogu-INRA cms in rapeseed (Brassica napus L.)
TLDR
This family of plants, R2000, showed a unique combination of molecular markers when compared to other rapeseed restorers analysed, which suggests that the recombination event allowed the recovery of B. oleracea genetic information that was originally replaced by the radish introgression in the original restorers.
The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats.
TLDR
A single radish nuclear gene, Rfo, restores Ogura (ogu) cytoplasmic male sterility (CMS) in Brassica napus and a radish gene encoding a 687-amino-acid protein with a predicted mitochondrial targeting pre-sequence was found to confer male fertility upon transformation into ogu CMS B. napus.
Transfer of resistance against the beet cyst nematode from radish (Raphanus sativus) to rape (Brassica napus) by monosomic chromosome addition
TLDR
The analysis in the cross to rape of a plant monosomic only for chromosome d confirmed the strong effect of this chromosome on nematode resistance, independent of the glucosinolate content in the roots.
Genetic characterization of a pentatricopeptide repeat protein gene, orf687, that restores fertility in the cytoplasmic male-sterile Kosena radish.
TLDR
It is proposed that ORF687 functions either directly or indirectly to lower the levels of ORF125, resulting in the restoration of fertility in CMS plants.
Development of a complete set of disomic rape-radish chromosome-addition lines
After crosses of multiple rape-radish chromosome additions with rape, nine different monosomic additions (2n = 4x = 38 + 1) of individual radish chromosomes, a–i, were isolated, having the genomic
Physical organization of the major duplication on Brassica oleracea chromosome O6 revealed through fluorescence in situ hybridization with Arabidopsis and Brassica BAC probes.
TLDR
It is shown that the physical order of corresponding genetic markers is highly conserved between the duplicated regions in B. oleracea and Arabidopsis and the physical lengths of the regions at pachytene are similar, while the genetic distances are considerably different.
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