Adenylate cyclase from the bovine cerebral cortex has been solubilized with the nonionic detergent Triton X-100. The physical properties of the enzyme have been determined. These are: sedimentation coefficient, 8.1 S; Stokes radius, 70 A; partial specific volume, 0.79 ml/g; mass, 305,000 daltons; f/f0 1.5. The high partial specific volume measured in the… (More)
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