Sitosterol-β-glucoside as Primer for Cellulose Synthesis in Plants

@article{Peng2002SitosterolglucosideAP,
  title={Sitosterol-$\beta$-glucoside as Primer for Cellulose Synthesis in Plants},
  author={Liangcai Peng and Y. Kawagoe and Pat Hogan and D. Delmer},
  journal={Science},
  year={2002},
  volume={295},
  pages={147 - 150}
}
Cellulose synthesis in plants requires β-1,4-glucan chain initiation, elongation, and termination. The process of chain elongation is likely to be distinct from the process of chain initiation. We demonstrate that a CesA glucosyltransferase initiates glucan polymerization by using sitosterol-β-glucoside (SG) as primer. Cotton fiber membranes synthesize sitosterol-cellodextrins (SCDs) from SG and uridine 5′-diphosphate–glucose (UDP-Glc) under conditions that also favor cellulose synthesis. The… Expand
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References

SHOWING 1-10 OF 20 REFERENCES
Unusual β-D-Xylosides That Prime Glycosaminoglycans in Animal Cells*
TLDR
Interestingly, benzyl-β-D-threo-pentopyranos-4-uloside (4-keto derivative) and benzyl -4-methyl- β- D-xyloside, with a methyl group in place of an axial hydrogen at C-4, primed GAG chains, which suggests the possibility of designing inhibitors of GAG synthesis based on xylosides with reactive groups in key positions. Expand
In vitro synthesis of a microfibrillar (1→3)-β-glucan by a ryegrass (Lolium multiflorum) endosperm (1→3)-β-glucan synthase enriched by product entrapment
TLDR
The product-entrapped enzyme preparation contains six major polypeptides, and comparison of the SDS—PAGE pattern of this fraction with thepolypeptide profile of an immunoprecipitated (1→3)-β-glucan synthase preparation suggests that polypePTides at 30–31 and 55–58 kDa are the most likely candidates for participation in (1–3)- β- glucan synthesis. Expand
The experimental herbicide CGA 325'615 inhibits synthesis of crystalline cellulose and causes accumulation of non-crystalline beta-1,4-glucan associated with CesA protein.
Developing cotton (Gossypium hirsutum) fibers, cultured in vitro with their associated ovules, were used to compare the effects of two herbicides that inhibit cellulose synthesis:Expand
Parallel-up structure evidences the molecular directionality during biosynthesis of bacterial cellulose.
The "parallel-up" packing in cellulose Ialpha and Ibeta unit cells was experimentally demonstrated by a combination of direct-staining the reducing ends of cellulose chains andExpand
CELLULOSE BIOSYNTHESIS: Exciting Times for A Difficult Field of Study.
  • D. Delmer
  • Chemistry, Biology
  • Annual review of plant physiology and plant molecular biology
  • 1999
TLDR
Genetic evidence now supports the concept that members of this family encode the catalytic subunit of the cellulose synthase in these organisms, with various members showing tissue-specific expression. Expand
Isolation and structure of diglycosylsterols and triglycosylsterols in rice bran
Abstract Novel oligoglycosylsterols, a series of sterylglycosides, were detected in the glycolipids of rice bran, and di- and triglycosylsterols were isolated and their structures were investigated.Expand
Chitin oligosaccharide synthesis by rhizobia and zebrafish embryos starts by glycosyl transfer to O4 of the reducing-terminal residue.
TLDR
The results indicate that prenylpyrophosphate-linked intermediates are not involved in the chitin oligosaccharide synthesis pathway, and shows that the use of artificial glycosyl acceptors such as pNPGlcNAc has not previously been described for a processiveglycosyltransferase. Expand
Role of the putative membrane-bound endo-1,4-beta-glucanase KORRIGAN in cell elongation and cellulose synthesis in Arabidopsis thaliana.
TLDR
It is demonstrated that the product of this gene is required for cellulose synthesis, which encodes a putative membrane-bound endo-1,4-beta-glucanase in KORRIGAN. Expand
PROCUSTE1 Encodes a Cellulose Synthase Required for Normal Cell Elongation Specifically in Roots and Dark-Grown Hypocotyls of Arabidopsis
TLDR
Mutants at the PROCUSTE1 (PRC1) locus show decreased cell elongation, specifically in roots and dark-grown hypocotyls, suggesting that cellulose synthesis in these organs requires the coordinated expression of at least two distinct cellulose synthase isoforms. Expand
Molecular Directionality of Polysaccharide Polymerization by thePasteurella multocida Hyaluronan Synthase*
  • P. Deangelis
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 1999
TLDR
It is shown here that a HA synthase, PmHAS, from Gram-negative P. multocida bacteria polymerizes the HA chain by the addition of sugar units to the nonreducing terminus, and the fidelity of the individual sugar transfer reactions is sufficient to generate the authentic repeating structure of HA. Expand
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