Sitosterol-β-glucoside as Primer for Cellulose Synthesis in Plants
@article{Peng2002SitosterolglucosideAP, title={Sitosterol-$\beta$-glucoside as Primer for Cellulose Synthesis in Plants}, author={Liangcai Peng and Yasushi Kawagoe and Pat Hogan and Deborah Pierson Delmer}, journal={Science}, year={2002}, volume={295}, pages={147 - 150} }
Cellulose synthesis in plants requires β-1,4-glucan chain initiation, elongation, and termination. The process of chain elongation is likely to be distinct from the process of chain initiation. We demonstrate that a CesA glucosyltransferase initiates glucan polymerization by using sitosterol-β-glucoside (SG) as primer. Cotton fiber membranes synthesize sitosterol-cellodextrins (SCDs) from SG and uridine 5′-diphosphate–glucose (UDP-Glc) under conditions that also favor cellulose synthesis. The…
342 Citations
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References
SHOWING 1-10 OF 20 REFERENCES
Unusual β-D-Xylosides That Prime Glycosaminoglycans in Animal Cells*
- Chemistry, BiologyThe Journal of Biological Chemistry
- 1996
Interestingly, benzyl-β-D-threo-pentopyranos-4-uloside (4-keto derivative) and benzyl -4-methyl- β- D-xyloside, with a methyl group in place of an axial hydrogen at C-4, primed GAG chains, which suggests the possibility of designing inhibitors of GAG synthesis based on xylosides with reactive groups in key positions.
In vitro synthesis of a microfibrillar (1→3)-β-glucan by a ryegrass (Lolium multiflorum) endosperm (1→3)-β-glucan synthase enriched by product entrapment
- Biology
- 1995
The product-entrapped enzyme preparation contains six major polypeptides, and comparison of the SDS—PAGE pattern of this fraction with thepolypeptide profile of an immunoprecipitated (1→3)-β-glucan synthase preparation suggests that polypePTides at 30–31 and 55–58 kDa are the most likely candidates for participation in (1–3)- β- glucan synthesis.
The experimental herbicide CGA 325'615 inhibits synthesis of crystalline cellulose and causes accumulation of non-crystalline beta-1,4-glucan associated with CesA protein.
- BiologyPlant physiology
- 2001
Developing cotton (Gossypium hirsutum) fibers, cultured in vitro with their associated ovules, were used to compare the effects of two herbicides that inhibit cellulose synthesis:…
Parallel-up structure evidences the molecular directionality during biosynthesis of bacterial cellulose.
- Chemistry, BiologyProceedings of the National Academy of Sciences of the United States of America
- 1997
The "parallel-up" packing in cellulose Ialpha and Ibeta unit cells was experimentally demonstrated by a combination of direct-staining the reducing ends of cellulose chains and…
CELLULOSE BIOSYNTHESIS: Exciting Times for A Difficult Field of Study.
- Biology, ChemistryAnnual review of plant physiology and plant molecular biology
- 1999
Genetic evidence now supports the concept that members of this family encode the catalytic subunit of the cellulose synthase in these organisms, with various members showing tissue-specific expression.
Chitin oligosaccharide synthesis by rhizobia and zebrafish embryos starts by glycosyl transfer to O4 of the reducing-terminal residue.
- Biology, ChemistryBiochemistry
- 1999
The results indicate that prenylpyrophosphate-linked intermediates are not involved in the chitin oligosaccharide synthesis pathway, and shows that the use of artificial glycosyl acceptors such as pNPGlcNAc has not previously been described for a processiveglycosyltransferase.
Role of the putative membrane-bound endo-1,4-beta-glucanase KORRIGAN in cell elongation and cellulose synthesis in Arabidopsis thaliana.
- BiologyPlant & cell physiology
- 2001
It is demonstrated that the product of this gene is required for cellulose synthesis, which encodes a putative membrane-bound endo-1,4-beta-glucanase in KORRIGAN.
Molecular Directionality of Polysaccharide Polymerization by thePasteurella multocida Hyaluronan Synthase*
- BiologyThe Journal of Biological Chemistry
- 1999
It is shown here that a HA synthase, PmHAS, from Gram-negative P. multocida bacteria polymerizes the HA chain by the addition of sugar units to the nonreducing terminus, and the fidelity of the individual sugar transfer reactions is sufficient to generate the authentic repeating structure of HA.
Characterization of a functional soluble form of a Brassica napus membrane-anchored endo-1,4-beta-glucanase heterologously expressed in Pichia pastoris.
- BiologyPlant physiology
- 2001
The functional analysis of Delta(1-90)Cel16, the N terminally truncated Cel16, missing residues 1 through 90 and comprising the catalytic domain of Cel16 expressed recombinantly in the methylotrophic yeast Pichia pastoris as a soluble protein, showed that Delta( 1-90)-hydrolyzed carboxymethylcellulose is a true endo-acting glucanase.