Sites of rDNA transcription are widely dispersed through the nucleolus in Pisum sativum and can comprise single genes.

@article{Thompson1997SitesOR,
  title={Sites of rDNA transcription are widely dispersed through the nucleolus in Pisum sativum and can comprise single genes.},
  author={William F. Thompson and Alison F. Beven and Brian Wells and Peter J. Shaw},
  journal={The Plant journal : for cell and molecular biology},
  year={1997},
  volume={12 3},
  pages={571-81}
}
Incorporation by RNA polymerases of BrUTP into both plant root tissue and isolated plant nuclei as a method for localization of the sites of transcription has been used. In this paper pea root tissue was used, and under the conditions employed, nearly all the incorporation occurs in the nucleolus, and thus must be catalysed by RNA polymerase I. Immunofluorescence and confocal microscopy shows that incorporation occurs in a pattern consisting of many small foci distributed widely through the… CONTINUE READING

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