Site-specific recombinatorics: in situ cellular barcoding with the Cre Lox system

  title={Site-specific recombinatorics: in situ cellular barcoding with the Cre Lox system},
  author={T. S. Weber and M. Dukes and D. Miles and S. Glaser and S. Naik and K. Duffy},
  journal={BMC Systems Biology},
  • T. S. Weber, M. Dukes, +3 authors K. Duffy
  • Published 2016
  • Computer Science, Biology, Medicine
  • BMC Systems Biology
  • BackgroundCellular barcoding is a recently developed biotechnology tool that enables the familial identification of progeny of individual cells in vivo. In immunology, it has been used to track the burst-sizes of multiple distinct responding T cells over several adaptive immune responses. In the study of hematopoiesis, it revealed fate heterogeneity amongst phenotypically identical multipotent cells. Most existing approaches rely on ex vivo viral transduction of cells with barcodes followed by… CONTINUE READING


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    Cre recombinase: The universal reagent for genome tailoring
    • 1,204
    • PDF
    In vivo generation of DNA sequence diversity for cellular barcoding
    • 25
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    • PDF
    Dissecting T cell lineage relationships by cellular barcoding
    • 106
    • PDF
    BAC Modification through Serial or Simultaneous Use of CRE/Lox Technology
    • 9
    • Highly Influential
    • PDF