Site-specific recombination promotes plasmid amplification in yeast.

  title={Site-specific recombination promotes plasmid amplification in yeast.},
  author={Fredric C. Volkert and James R. Broach},
  volume={46 4},
All stable, naturally occurring circular yeast DNA plasmids contain a pair of long, nontandem inverted repeats that undergo frequent reciprocal recombination. This yields two plasmid inversion isomers that exist in the cell in equal numbers. In the 2 mu circle plasmid of S. cerevisiae such inversion is catalyzed by a plasmid-encoded site-specific recombinase, FLP. We show that the site-specific recombination system of 2 mu circle enables the plasmid to increase its mean intracellular copy… CONTINUE READING

From This Paper

Topics from this paper.


Publications citing this paper.
Showing 1-10 of 49 extracted citations

Similar Papers

Loading similar papers…