Site-specific recombination-based genetic system for reporting transient or low-level gene expression.

@article{Casavant2002SitespecificRG,
  title={Site-specific recombination-based genetic system for reporting transient or low-level gene expression.},
  author={N. Carol Casavant and Gwyn A. Beattie and Gregory J. Phillips and Larry J. Halverson},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 7},
  pages={3588-96}
}
We report here the construction, characterization, and application of a plasmid-based genetic system that reports the expression of a target promoter by effecting an irreversible, heritable change in a bacterial cell. This system confers strong repression of the reporter gene gfp in the absence of target promoter expression and utilizes the site-specific recombination machinery of bacteriophage P22 to trigger high-level reporter gene expression in the original cell and its progeny after target… CONTINUE READING

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