Site-specific photocrosslinking to probe interactions of Arf1 with proteins involved in budding of COPI vesicles.

@article{Fischer2000SitespecificPT,
  title={Site-specific photocrosslinking to probe interactions of Arf1 with proteins involved in budding of COPI vesicles.},
  author={Kathrin Fischer and Jeffrey B. Helms and Liyun Zhao and Felix Theodor Wieland},
  journal={Methods},
  year={2000},
  volume={20 4},
  pages={455-64}
}
ADP-ribosylation factor 1 (Arf1) plays an important role in early and intra-Golgi protein trafficking. During this process, Arf1 interacts with many different proteins and other molecules that regulate its state of activation or are involved in its intracellular function. To determine which of these proteins interact directly with Arf1 during coat protein type I (COPI) vesicle biogenesis, we probed the molecular environment of Arf1 by use of site-specific photocrosslinking. This method was… CONTINUE READING

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