Site-specific mutagenesis of residues in the human C5a anaphylatoxin which are involved in possible interaction with the C5a receptor.

@article{Bubeck1994SitespecificMO,
  title={Site-specific mutagenesis of residues in the human C5a anaphylatoxin which are involved in possible interaction with the C5a receptor.},
  author={P Bubeck and Joachim Groetzinger and M Winkler and J{\"u}rgen K{\"o}hl and Axel Wollmer and Andreas Klos and Wilfried Bautsch},
  journal={European journal of biochemistry},
  year={1994},
  volume={219 3},
  pages={
          897-904
        }
}
  • P Bubeck, Joachim Groetzinger, +4 authors Wilfried Bautsch
  • Published in
    European journal of…
    1994
  • Medicine, Biology
  • To check and clarify existing data on receptor-interacting residues in the human C5a anaphylatoxin, we tested mutant C5a proteins obtained by site-directed mutagenesis of a recombinant human C5a (rhC5a) cDNA clone for structural and functional integrity. Amino acid positions in three different regions of the molecule were investigated: Arg74 at the C-terminus, Arg40 and Pro45 located in the core region, and Lys14 and Lys19, Lys20 in the N-terminus. Des-Arg74-rhC5a displayed only a residual 3-4… CONTINUE READING

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