Site-specific interaction of thrombin and inhibitors observed by fluorescence correlation spectroscopy.

@article{Klingler1997SitespecificIO,
  title={Site-specific interaction of thrombin and inhibitors observed by fluorescence correlation spectroscopy.},
  author={J{\"u}rgen Klingler and Thorsten Friedrich},
  journal={Biophysical journal},
  year={1997},
  volume={73 4},
  pages={2195-200}
}
We report on the application of fluorescence correlation spectroscopy (FCS) to observe the interaction between thrombin and thrombin inhibitors. Two site-specific fluorescent labels were used to distinguish between inhibitors directed to the active site, the exosite, or both binding sites of thrombin. For several well-known inhibitors of thrombin, the binding sites observed by FCS correspond to previous studies. The interaction of the recently discovered thrombin inhibitor ornithodorin from the… CONTINUE READING