Site-specific integration in mammalian cells mediated by a new hybrid baculovirus-adeno-associated virus vector.

@article{Palombo1998SitespecificII,
  title={Site-specific integration in mammalian cells mediated by a new hybrid baculovirus-adeno-associated virus vector.},
  author={Fabio Palombo and A Monciotti and Alessandra Recchia and R. Cortese and G. Ciliberto and Nicola La Monica},
  journal={Journal of virology},
  year={1998},
  volume={72 6},
  pages={5025-34}
}
Baculovirus can transiently transduce primary human and rat hepatocytes, as well as a subset of stable cell lines. To prolong transgene expression, we have developed new hybrid vectors which associate key elements from adeno-associated virus (AAV) with the elevated transducing capacity of baculovirus. The hybrid vectors contain a transgene cassette composed of the beta-galactosidase (beta-Gal) reporter gene and the hygromycin resistance (Hygr) gene flanked by the AAV inverted terminal repeats… CONTINUE READING