Site-specific integrase-mediated transgenesis in mice via pronuclear injection.

@article{Tasic2011SitespecificIT,
  title={Site-specific integrase-mediated transgenesis in mice via pronuclear injection.},
  author={Bosiljka Tasic and Simon Hippenmeyer and Charlene Wang and Matthew Gamboa and Hui Zong and Yanru Chen-Tsai and Liqun Luo},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2011},
  volume={108 19},
  pages={7902-7}
}
Microinjection of recombinant DNA into zygotic pronuclei has been widely used for producing transgenic mice. However, with this method, the insertion site, integrity, and copy number of the transgene cannot be controlled. Here, we present an integrase-based approach to produce transgenic mice via pronuclear injection, whereby an intact single-copy transgene can be inserted into predetermined chromosomal loci with high efficiency (up to 40%), and faithfully transmitted through generations. We… CONTINUE READING

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