Site-specific incorporation of the mucin-type N-acetylgalactosamine-alpha-O-threonine into protein in Escherichia coli.

@article{Xu2009SitespecificIO,
  title={Site-specific incorporation of the mucin-type N-acetylgalactosamine-alpha-O-threonine into protein in Escherichia coli.},
  author={Ran Xu and Sarah R. Hanson and Zhiwen Zhang and Yu-ying Yang and Peter G. Schultz and C. -H. Wong},
  journal={Journal of the American Chemical Society},
  year={2009},
  volume={131 38},
  pages={13883}
}
To better accommodate the frequent requests for reagents to genetically incorporate unnatural amino acids into proteins, the expression vectors and conditions for a large number of distinct amino acids have now been standardized for Escherichia coli and Pichia pastoris. Regrettably, we have found that for the isolated case of glycosylated amino acids we are unable to incorporate them under these conditions or those originally described (and note complexities with nonsense suppression and… CONTINUE READING

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