Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells.

@article{Sakamoto2002SitespecificIO,
  title={Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells.},
  author={Kensaku Sakamoto and Akiko Hayashi and Ayako Sakamoto and Daisuke Kiga and Hiroshi Nakayama and Akiko Soma and Takatsugu Kobayashi and Makoto Kitabatake and Koji Takio and Kazuki Saito and Mikako Shirouzu and Ichiro Hirao and Shigeyuki Yokoyama},
  journal={Nucleic acids research},
  year={2002},
  volume={30 21},
  pages={4692-9}
}
A suppressor tRNA(Tyr) and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-L-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNA(Tyr) gene was mutated, at three positions in the D arm, to generate the internal promoter for expression. However, this tRNA, together with the cognate TyrRS, failed to exhibit suppressor activity in mammalian cells. Then, we found that amber suppression can occur with the heterologous pair of E.coli… CONTINUE READING
56 Citations
32 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 56 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 32 references

Similar Papers

Loading similar papers…