Site-specific enzymatic polysialylation of therapeutic proteins using bacterial enzymes.

@article{Lindhout2011SitespecificEP,
  title={Site-specific enzymatic polysialylation of therapeutic proteins using bacterial enzymes.},
  author={Theresa Lindhout and Umar Iqbal and Lisa M Willis and Anne N Reid and Jianjun Li and Xin Liu and Maria Flores Vizcaya Moreno and Warren W. Wakarchuk},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2011},
  volume={108 18},
  pages={7397-402}
}
The posttranslational modification of therapeutic proteins with terminal sialic acids is one means of improving their circulating half-life, thereby improving their efficiency. We have developed a two-step in vitro enzymatic modification of glycoproteins, which has previously only been achieved by chemical means [Gregoriadis G, Jain S, Papaioannou I, Laing… CONTINUE READING