Site-specific discrimination of Cytosine and 5-methylcytosine in duplex DNA by Peptide nucleic acids.

@article{Okamoto2002SitespecificDO,
  title={Site-specific discrimination of Cytosine and 5-methylcytosine in duplex DNA by Peptide nucleic acids.},
  author={Akimitsu Okamoto and Kazuhito Tanabe and Isao Saito},
  journal={Journal of the American Chemical Society},
  year={2002},
  volume={124 35},
  pages={10262-3}
}
For site-specific discrimination of cytosine (C) and 5-methylcytosine (mC) in duplex DNA, we developed a new method using peptide nucleic acids (PNAs). The combination of a PNA-assisted DNA displacement complex and a fluorescein-labeled probe oligomer allowed the detection of mC at the defined sites in target DNA using a restriction enzyme. After treatment of the complex with a restriction enzyme, strong fluorescence emission was observed for the complex containing C at the target site, whereas… CONTINUE READING