Site-specific chemical labeling of mitochondrial respiratory complex I through ligand-directed tosylate chemistry.

@article{Masuya2014SitespecificCL,
  title={Site-specific chemical labeling of mitochondrial respiratory complex I through ligand-directed tosylate chemistry.},
  author={Takahiro Masuya and Masatoshi Murai and Kentaro Ifuku and Hironobu Morisaka and Hideto Miyoshi},
  journal={Biochemistry},
  year={2014},
  volume={53 14},
  pages={2307-17}
}
The site-specific chemical modification of NADH-quinone oxidoreductase (complex I) by various functional probes such as fluorophores and microbeads, without affecting the enzyme activity, may allow single-molecule analyses of putative dynamic conformational changes in the enzyme. In an attempt to address this challenge, we performed site-specific alkynylation of complex I in bovine heart submitochondrial particles by means of a ligand-directed tosylate (LDT) chemistry strategy with synthetic… CONTINUE READING

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On the mechanism of respiratory complex I.

Journal of bioenergetics and biomembranes • 2014
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